
After being sucked into a drain pipe, Mario and Luigi defend the Mushroom Kingdom from King Koopa.

In the Amazon Jungle, Mario, using a plunger to swat his way through a variety of flora, leads Luigi, Toad and Princess Toadstool in their search for Dr Sheldon, a mysterious witch doctor.

In the Snow World, Mario, Luigi, Toad and Princess Toadstool, exhausted and hungry, weakly venture forth through a snowy field, mentioning that the hordes of Koopa Khan are after them.

In the badlands of Car Land, Mario, Luigi, Princess Toadstool and Toad ride in a brown convertible car, and plan on rendezvousing with several Mushroom rebels.

In the ancient city of Mythis, Mario, Luigi, Toad, and Princess Toadstool are searching for a legendary hero named Hercufleas, who is an old friend of Toad's.